I want to check several genes in one single cell, What kit can i use?
1.I want to isolate brown adipose from mice. I am not sure whether the tissue is right？（show in the attachment.) 2. I want to digest brown adipose to collected single cell sample. After 1 hour...
03 April 2018 2,783 0 View
I want to find the list of mice receptor ligand binding.
02 March 2018 6,109 3 View
1. western and RT PCR show the protein is high expression. 2.FCM show opposite result. 3.The antibody is different. ^^^^^The protein have been modified after translate?
11 December 2016 2,310 3 View
I want to do Ex Vivo Functional Analysis of human Dendritic Cells. What i found was here: 1,Measurement of Export to the Cytosol in Dendritic Cells Using a Cytofluorimetry-Based Assay (Principal...
09 October 2016 342 1 View
I got some NSG-like mice from company(in China). Those mice can survival in lab gangway（not clear） for almost 2 weeks. Does it normal? I want to check their blood through FCM. And I want to use...
08 September 2016 7,228 3 View
Read 3 answers by scientists with 1 recommendation from their colleagues to the question asked by Hyde Chen on Sep 21, 2016
08 September 2016 9,749 3 View
I use the 4034 from stemcell. Seed cell are cd34+ from hES.
06 July 2016 4,462 4 View
I designed many primers and they will work as a mixture. I am not sure whether those primers bind with each other (instead of template). I try to find some soft to check the quality of those...
06 July 2016 8,578 4 View
HSC are Cryopreserved. After thawing, HSC are used directly or culture on SFEM for a while before bone marrow transplantation（human HSC to mice）？ Does Cryopreserving affect the activity of HSC.
06 July 2016 1,228 1 View
A friend has a patient with a new mutation (leukemia). He want to keep this mutation to further study. So he collected monocyte from patient and want to make a immortal cell line. He do not know...
05 June 2016 2,010 5 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
