How to check the rearrangement of human T and B cells? If using PCR what is the primer or kit?

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Kai Wong

Do you mean T cell and B cell gene rearrangement?

Hyde Chen

Sorry, My question only have title (the web problem in China or University- the content is Upload failed)。 Yes，i want to collect the T and B cell from blood and check their

Piero Pileri

you can use inverse PCR ..

Immunogenetics. 1991;34(3):174-8.

A novel and rapid cloning method for the T-cell receptor variable region sequences.