Add Complete med. above my cell agar layer?

Alice Gentil Dit Maurin @Alice-Gentil-Dit-Maurin

06 July 2016 1 1K Report

Hi all,

I was wondering why my positive control (NIH/3T3 + RasG12V transfection) doesn't form colonies on my soft agar assay...

Just after the gelation of the second layer (cell/medium/agar, 15 min 4C), I add 100ul of complete medium...

Do you think this could impact on my colonies formation?

Thanks a lot in advance, :)

Alice

Alice Gentil Dit Maurin

Sorry I don't think that my problematic can be related to this article.

It s not a soft agar assay. Just agar plates to grow this type of bacteria.

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Science method

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