8 Questions 16 Answers 0 Followers
Questions related from Alice Gentil Dit Maurin
Hello everyone. Question when you are developing stable cell line in CHOK1 parental cell. I want to have a pure cell population expressing a specific receptor quantity at the cell surface (nice...
18 May 2021 6,625 5 View
Hello, Is someone know if it's possible from a crystallized protein modelisation to know the distance between X and Y amino acid? Which softwares/ websites to consider? Thanks a lot in...
06 June 2019 8,958 4 View
Hello there! I am struggling using cyclic nucleotides from InVivogen (c-di-AMP, and 2'3'-cGAMP) on my THP1 cells. I use the nucleotides directly on my cells and I have really weird results (high...
23 February 2017 1,664 2 View
Hello everybody, I have some issues with my pharmacology study. I have different EC50 for one compound (one of my cyclic nucleotides) between frozen and fresh cells... It's like it's more active...
17 February 2017 5,859 4 View
Hello everyone, I was wondering which type of serum I can use for a phagocytosis assay (human macrophages/mouse microglia)... in particular if the non heat inactivated serum (so with complement)...
06 December 2016 3,536 8 View
Hi all, I was wondering why my positive control (NIH/3T3 + RasG12V transfection) doesn't form colonies on my soft agar assay... Just after the gelation of the second layer (cell/medium/agar, 15...
06 July 2016 1,234 1 View
Hi All, I am starting to do some soft agar assays using NIH/3T3 cells. As positive control I used NIH transfected with RasG12V plasmid. Before placing my cell on the agar gel I noticed (twice and...
01 July 2016 6,427 1 View
Does anyone work on nanoparticles (cyclodextrin)? How in vitro cytotoxicity is about positive charged particles (with Stearylamin)? Mine are really toxic on HeLa cell (10 ug/ml) : under microscope...
24 April 2015 1,846 2 View
