Roba Alatawy

2 Questions 14 Answers 0 Followers

Questions related from Roba Alatawy

PCR gene amplification with transposon insertion?

I have a primer pair which i use to amplify the wild type allele. I tried to use the same pair to amplify the mutated gene with a tranposon insertion. I see no amplification at all. Does any body...

05 May 2016 7,065 4 View

The infusion cloning kit is not working for me?

I use this kit trying to clone a gene 1011 bp in size into a shuttle vector 6000 bp. I got transformants on the plates but after the screening using either sequencing or colony PCR nothing there...

03 March 2016 8,211 5 View