Hello,
I am trying to measure caspase activity in CHO cell samples using the Flurochrome-labeled inhibitor of caspases assay (FLICA). I want to look at ethanol (or another checmical) induced apoptosis.
Does anybody have a protocol for this. Or could let me know for how long to leave the CHO sample in ethanol and what percentage.
I was originally trying to use heat- however we are not picking up any caspase activity in the sample.
Using Chemometic NC-3000
Thank you!