25 March 2022 2 6K Report

Hello,

I am trying to measure caspase activity in CHO cell samples using the Flurochrome-labeled inhibitor of caspases assay (FLICA). I want to look at ethanol (or another checmical) induced apoptosis.

Does anybody have a protocol for this. Or could let me know for how long to leave the CHO sample in ethanol and what percentage.

I was originally trying to use heat- however we are not picking up any caspase activity in the sample.

Using Chemometic NC-3000

Thank you!

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