Whats wrong with the Illumina sequencing?

More Sushmitha T J's questions See All

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Has anyone used the Illumina ITS1 primer sets?

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Errors in DNA sequences?

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Can I use FLEXBAR to detect and remove barcodes out of my short reads without demultiplexing ?

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22 February 2021 9,873 3 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

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What is the significance of (isolated) high titers of IgG anti GD1a antibodies in an older patient with polyneuropathy?

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Repository for capture-seq raw reads?

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Unable to detect a truncated protein variant, but I can observe a truncated cDNA from Sanger?

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Sergio Alías-Segura

I would say there is nothing to worry about. Check https://www.biostars.org/p/9490605/

Sushmitha T J

Dear Sergio, thank you.

Abhijeet Singh

Looks like it is just one read. How many reads do you have for that sample?

Total Sequences: 32045184

Then you should just proceed with the next step in your analysis. You can't do anything anyway to change the quality of your data. But, the data is very good, probably due to the short read length. Conclusion, there is nothing to worry about.

Kuberan Ganesan

The fastqc data looks good quality-wise.