I am currently doing cell culture on Wharton -Jelly Mesenchymal Stem Cell. Is anyone know whether 10nM or 100nM of dexamethasone is the best for osteogenic differentiation.Your help is highly appreciated.
Really appreciated your respons. It means that you can see the osteogenic differentiation within 1 week.?By the way, Thank you for your answer.
Really appreciated your respons. It means that you can see the osteogenic differentiation within 1 week.?By the way, Thank you for your answer.
Really appreciated your respons. It means that you can see the osteogenic differentiation within 1 week.?By the way, Thank you for your answer.
Really appreciated your response. It means that you can see the osteogenic differentiation within 1 week.?By the way, Thank you for your answer.
I haven't tried it with primary MSCs, but I used to use 100nM for C3H10T1/2 cells. Cells seemed to differentiate well within the first week, but when I expanded the culture period from 2-3 weeks, I observed many adipocyte-like cells, which were positive for oil red.
I hope this article could help: http://www.ncbi.nlm.nih.gov/pubmed/25887471
Hello
I use 100nM with my cells .
http://www.ecmjournal.org/journal/papers/vol023/pdf/v023a02
100nM seems to be optimal to direct MSC toward osteogenic differentiation. I was used the same concentration for hMSC towards osteogenic differentiation and quantification of Ca++ accumulation was performed by Alizarin Red staining which is very convenient in this context.
For osteogenic differentiation of MSCs, we cultured the cells in DMEM medium supplemented with 100nM of dexamethasone and we saw the evidence of osteogenic differentiation within a week.
Thank you all for the answer and explanation. Really appreciated it. Hopefully my work went well till end of my project. Have a good day to all
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