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I have been so far to load a raw mzxml chromatogram file which I generated using Data analysis software, and I also tried using mzxml file from Mascot, but I didn't succeeded. The software always...
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Does the degree of denaturation affects the digestion processalong with the lc-ms/ms result? If so then how does it affects?
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I've been using this protocol but i'm unable to get back the maximum protein yield in Nano ESI Q TOF LC-MS/MS The pipeline goes this way: 1. cell line lysis and homogenization 2. acetone...
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Generally, it is performed after Reduction and Alkylation steps. But if trypsin digestion is performed prior to SDS PAGE, will it affect the peptide coverage? And how? Since we performed that way...
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