How can I prepare 1 L tris-HCL 0.1 mol/L buffer?
Weigh out 12.114g of Tris, add that to 800mL of water, use HCl to reach desired pH, and top off with water to 1 L.
Hello, I am performing statistical analysis of my research data by comparing the mean values by using Tukey HSD test. I got homogeneous group in both small and capital alphabets. This is because...
07 August 2018 6,422 9 View
Hello, I am preforming research to check the impact of different storage bags on the damage parameters of maize due to red flour beetle at different storage times. Treatments: 8 storage bags...
07 August 2018 9,021 4 View
Hello everyone I want to know is Na2SiO3.9H2O soluble in only water or water+HCl. Thanks
08 September 2016 6,929 4 View
Hello everyone, I studied some research papers that soil pH will be changed after the addition of sodium metasilicate. So many of them used 0.01N H2SO4 for adjusting pH under laboratory and...
08 September 2016 2,167 3 View
Hello everyone I want to perform a prelimainary experiment for checking the resistance of six wheat varieties against wheat aphid under laboratory condition. For this I will sow 10 wheat seeds of...
07 August 2016 7,380 4 View
Hello, I have selected 2 different fields for silicon determination from soil. These field are present on the same location. Two soil samples (top soil and sub soil) are taken from each field for...
07 August 2016 3,022 5 View
Hello everyone, I want to dissolve calcium silicate (CaSiO3) in water and then applied to wheat plant, but according to literature it is insoluble in water. So kindly tell me that method which...
07 August 2016 4,013 3 View
Hello, I want to determine the available silicon concentration (g/kg) in the soil by using gravimetric method. Can anyone please tell me the complete procedure how to use this method?
04 May 2016 7,290 2 View
Clip cages are used in laboratories for biology determination of insects. Can anyone please tell me the best way to find out the biology of small insects like wheat aphids in the field condition?
02 March 2016 4,990 3 View
I have 1 liter calcium silicate solution and want to prepare ten different concentrations of the silicate to treat wheat plants under laboratory condition by soil and foliar application. Can...
02 March 2016 9,916 4 View
Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?
07 August 2024 4,616 0 View
Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...
06 August 2024 5,373 2 View
Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...
05 August 2024 3,783 2 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
Hello everyone! How can i prepare EDTA buffer solution (pH 8.2) to determine GSH levels? Thank you in advance.
29 July 2024 4,374 1 View
I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.
29 July 2024 8,560 0 View
// interested in the difference between floating events and short circuits.
22 July 2024 6,565 0 View
Our lab has extracted bacterial DNA samples using Qiagen DNeasy kit and eluted the samples in AE buffer. However, this buffer contains 0.5mM EDTA not suitable for downstream application. Any ideas...
13 July 2024 1,675 3 View
Commonly, we prepare 1.0% of agarose gel with 0.4g agarose powder and 40ml 1X TAE buffer. if I would like to prepare a 1.2%% of agarose gel, is it I need to add 0.48g agarose powder and 120ml 1X...
12 July 2024 2,058 5 View
I performed CV of Cu-MOF in a buffer solution of pH 8, and we did not find any peak corresponding to Cu2+ to Cu+. What is the reason behind this? Please give your valuable suggestions.
08 July 2024 2,388 4 View