Eden Tanzosh

3 Questions 3 Answers 0 Followers

Questions related from Eden Tanzosh

Does anyone have experience transfecting a plasmid designed for in vitro transcription/translation into insect cells (Sf9/21)?

I used the pF25K ICE T7 Flexi Vector from Promega with the T7 in vitro transcription/translation kit for small scale protein expression. Now I need to scale up my expression and my PI suggested...

06 June 2016 4,297 1 View

How to increase SDS-PAGE band sharpness?

Hello, I'm having a problem with blurry bands on SDS-PAGE. Does anyone have any suggestions as to how to increase the sharpness of banding? I have attached an example of my gels which I silver...

12 December 2015 1,871 12 View

Why is my SDS-PAGE marker not resolving all the way?

Hello everyone. I need some help troubleshooting my SDS-PAGE. My marker seems to not be resolving fully with the last band coming out being 40kDa. I have attached a picture as an example. My...

11 November 2015 2,933 1 View