Hi, I am working with TRBO-G vector with the size of 11.2kb, when i am doing plasmid isolation, it showing single band structure in agarose gel.
can anyone help me to solve the problem with this
Can i confirm that as my plasmid and proceed with my work ?
If the size of the plasmid is correct (you should digest it with an enzyme that cuts one time) then it suggests you are likely to have the right plasmid. You could do some predictive digests based on your map of the plasmid to confirm that it is the right one.
HOWEVER, just because the size is right doesn't mean it is 100% correct, it would be best if you sequenced the plasmid (or certainly the important regions) to be sure there are no mutations or someone didn't send you a similar but not correct plasmid. If you intend to do a lot of work with this plasmid, you don't want to lose a year and then discover there was a problem.
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