What are some possible substrates that can be used to test laccase activity?

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What is meant by Hits (DB) in High-Resolution Liquid Chromatography-Mass spectrometry (HR-LCMS) analysis compound table report?

In the qualitative compound report obtained from HR-LCMS analysis of crude plant extracts, what is meant by Hits (DB)? Should we consider all the predicted compounds from the list for further studies?

30 June 2024 2,290 4 View

Can 0.1% ammonium hydroxide damage HILIC column?

In my LC-MS experiment, I am utilizing an Agilent Polaris NH2 column to separate a mixture of glucose, fructose, and sucrose. I am using water and acetonitrile as A and B mobile phases...

26 March 2024 8,895 4 View

Can ProSA web server be used to analyze the protein structures downloaded from Protein Data Bank?

Or is it used only to analyze the modelled protein structures.

02 March 2024 6,662 2 View

How to visualize interactions of protein-protein docked model obtained from ClusPro web server?

Not able to view the downloaded model from ClusPro using PDBSum. Since both the docked proteins contain single A chain, PDBSum shows it as single image. Please give suggestions. Also tried to save...

18 January 2024 5,286 4 View

Why polymer like peaks with C30 column always?

I am using a C30 column for carotenoid analysis. I bought the column recently and using it for the first time. When I use the column in LC-MS scan mode, I clearly see the UV-Vis peaks for my...

08 June 2023 2,357 2 View

What is the possible peak at m/z = 535.8 for lycopene?

I am analyzing Lycopene using LC-MS. I use a C18 column and my mobile phase is Methanol: MTBE = 95:5. I use an Agilent Ultivo QQQ mass spectrometer with APCI negative ion mode. I see the precursor...

07 May 2023 525 0 View

What does "defined levels are outside of dataset range" mean in 2D NMR?

I am running an HMBC experiment on a Bruker 300 mHz instrument. I set up the experiment following the steps given in TopSpin guide book. Everything happens fine but when I try to open the spectrum...

09 March 2023 5,289 4 View

What is a suitable solid phase extraction method for fatty acid methyl esters?

I need to concentrate some fatty acid methyl esters in a cell culture medium to separate and isolate them using Prep-HPLC. What type of solid phase extraction would be suitable to concentrate...

02 March 2023 9,526 0 View

Is it possible to use the same column for HPLC and LC-MS?

I used the following column for an HPLC separation; C18 4.6 x 250 mm, 5 um, 120 Ao. I used methanol and acetonitrile as mobile phases. Now I have to do LC-MS for the same samples. I am wondering...

19 December 2022 1,889 6 View

Which markers are best to perform FACS staining for macrophage from PBMC in Human?

Hello, Can anyone please suggest the markers and protocol for FACS staining for macrophage from PBMC in human. Thank you.

09 March 2022 642 8 View

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Why might the impedance values for DI water and 0.1X PBS buffer solution exhibit a decreasing and increasing trend, respectively over time (HP 4194A)?

Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...

05 August 2024 3,783 2 View

Can a Conventional 50W visible LED Lamp Emit UV Light?

I'm researching light sources and their UV emissions. I'm curious if a 50W, 7500K conventional LED lamp emits any UV light. I found that a 200W tungsten lamp can emit up to 1.5 mW/cm² at 350 nm....

31 July 2024 3,843 7 View

Mossbauer spectroscopy Co0.6Mn0.4Fe2O4 MNPs?

Following curve related to Co0.6Mn0.4Fe2O4 Magnetic nanoparticles with extra phase a-Fe2O3. I will appreciate to help me to interpretation this spectroscopy, especially the blue line.

31 July 2024 9,140 2 View

How to calculate conductivity using Electrochemical Impedance Spectroscopy's Nyquist plot?

Hello everyone, I am new to this characterization but watching videos on youtube I have already plotted a Nyquist plot (Z' and -Z'') and already fitted models on my material using Zsim. But now I...

29 July 2024 1,376 0 View

How can we calculate the percentage of configuration interaction (CI) in the UV output data of the Gaussian program?

How can we calculate the percentage of configuration interaction (CI) in the UV output data of the Gaussian program? for example: Excited State 17: Singlet-A 5.1359 eV 241.41 nm...

28 July 2024 9,165 2 View

Why semicircle of nyquist plot is not complete?

While I am doing HER, I noticed that nyquist plots of Electrochemical Impedance Spectroscopy (EIS) is not complete. Will it affect for the Linear Sweep Voltametry (LSV) data? However, I can found...

26 July 2024 7,542 4 View

What is single absorption ESR spectroscopy ?

In the context of Electron Spin Resonance (ESR) spectroscopy, under what conditions is an absorption spectrum considered to be a single absorption spectrum?

25 July 2024 2,325 1 View

Can we fit a gaussian Fit on a LIBS Peak ?

Though much literature suggests fitting a Voigt profile on a LIBS peak, is it also possible to fit a Gaussian profile on the LIBS peak? I have 204 spectra files, and I am building a pipeline for...

23 July 2024 6,127 1 View