Is this degradation or 5S band? | ResearchGate

01 January 1970 0 8K Report

Hi there - I've been trying to optimise total RNA extraction from bacterial cultures, but I keep getting RIN = N/A on the Agilent Bioanalyser and a very sharp peak on the trace (about 25-30s). I still have 2 very distinct 16S and 23S bands. Is this degradation or is this a small RNA peak? Any advice would be greatly appreciated. Thanks in advance!

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