I am trying to grow bacterial cells in human plasma to test for sRNA differential expression. But the company I bought plasma from said that the complement degrades almost immediately and that my experimental design is flawed. I want to know just how quickly it degrades when thawed on ice after being frozen in -80. Thus far I haven't found anything. If anyone knows how to extend complement life or has any ideas, please let me know! Thanks!
Disclaimer: I am using Innovative Research to buy plasma and they have been a great company to interact with- they just can't answer my questions in this regard.
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0049147
Complement proteins have short half-life both in vivo and in vitro (this is on of the potential problems associated with complement measurement) and different complement have different half-life....
http://www.hindawi.com/journals/jir/2012/962702/
https://books.google.iq/books?id=zHAoDwAAQBAJ&pg=PA72&lpg=PA72&dq=How+quickly+do+complement+proteins+degrade+in+human+plasma&source=bl&ots=UyMARPa4am&sig=aFeSjDKd5AKT1HcNpHlA4CWxjl4&hl=ar&sa=X&ved=0ahUKEwjGnaKRy5rVAhWEalAKHbI7AIE4ChDoAQgsMAE#v=onepage&q=How%20quickly%20do%20complement%20proteins%20degrade%20in%20human%20plasma&f=false.
Purification of Complement Components,
Regulators, and Receptors by Classical Methods.
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- Chemistry
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- Small RNA