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I have 200 samples, each one has measurements of the same 600 proteins. The samples are from 3 groups: before cancer, after cancer, no cancer. I want to find the right proteins, any combination of...
22 November 2019 3,740 3 View
Hi everyone, I am running a linear mixed model in R, using the lme-function. The following variables are used: Y ... Outcome (continuous) IV1 ... Time (categorical: 0, 1, 2, 3) IV2 ... Group...
14 November 2019 4,072 7 View
Can I ask, why Streptomyces sp. is very sensitive and easily contaminated even when doing glycerol stock? After I activate back my stock, it was found contaminated after 1 day. I have sterilize...
03 November 2019 828 3 View
Hello. I am looking for a solution in R to apply spatially-constrained clustering using fuzzy K-means (fuzzy c-means). I came across the spatialClust package, and was wondering whether anyone may...
31 October 2019 4,963 2 View
Hi everyone, I have some issues to determine how to pick correctly the right distance calculation method in R. I have two datasets : one containing habitat variables (worldclim data, so...
16 October 2019 7,816 5 View
I am trying to check if my sample characteristics are similar to the population characteristics for the following 2 cases. 1. I have the information regarding the gender distribution of my...
13 October 2019 342 14 View
A lot of the mRNA data that I get is already normalized and getting to the normalized counts is not trivial. While I'd prefer to use R packages like DeSeq2 or edgeR, they use raw counts. Is there...
10 October 2019 6,019 4 View
Hello People I have a set with two groups of snakes I want to compare, but there are also cofactors (e. g. sex, habitat ecc). Then I should use ANCOVA according to your answers. But I have also...
10 October 2019 8,865 5 View
Hi，everyone I have some problem when I calculate the mean effect size. Suppose that the A parameter has ten data from ten articles. How should I synthesize the data and calculate a total effect...
10 October 2019 4,048 4 View
I am currently trying to edit the Corine Land Cover (CLC2018) raster map of Europe. I need to condense the landcover classes into a smaller number of different classes and then convert those...
10 October 2019 8,973 2 View
Dear all, Can anyone tell me how to fit nonlinear mixed models by the first-order (FO) and first-order conditional expectation (FOCE) methods ? I only know that a Package called nlme can be used...
10 October 2019 1,290 3 View
10 October 2019 9,371 6 View
Dear all, I have a huge database of crude hourly meteorological data, and I am looking for some tutorials for processing it, filling gaps, detect outliers, etc. preferably using R (but I could...
10 October 2019 9,121 6 View
Hello, everyone. I am studying the influence of one biomarker on multiple disease characteristics, and I would like to calculate its cutoff point. I created univariate ROC curves to investigate...
10 October 2019 9,800 4 View
I have performed a mixed model logistic regression using glmer and used afex::mixed to obtain the correct pvalues. mixed(model_9, data = ReversalTaskData, method = "PB", test_intercept = T,...
10 October 2019 10,059 3 View
Hi, I'm analyzing a dataset with the yearly concentration indexes of 50 different sectors (e.g., automobiles, pharmaceuticals, etc.) of a specific country from 1996 until 2016. However, I...
10 October 2019 2,889 4 View
10 October 2019 8,739 3 View
I'm a novice to RNA-seq analysis and DGE, hoping to get some good ideas moving forward. For context: I have 15 samples, 3 replicates each of 5 sample types. They're mRNA samples obtained from...
10 October 2019 8,397 4 View
08 October 2019 8,291 3 View
I'm currently using R to analyze some metabolomics data, and I'm using the hmdbQuery package to download HMDB entries for each metabolite in my dataset. I'm having some trouble working with the...
07 October 2019 10,237 1 View
Hello everybody, I have a dataset with 8 predictors (dimensions of brand image) that I regress on 4 different DVs (Differentiation, Relevance, Esteem and Knowledge). One DV at a time. In order...
04 October 2019 4,881 5 View
Hi, I am running excitation-emission fluorescence spectra (on algal samples) and I want to use the EEM R package to visualize and analyze the data. However, this package only supports data files...
27 September 2019 2,137 2 View
I am currently trying to estimate the relationship between two variables, let’s call them X and Y. To better understand how those two variables are correlated across studies and between studies, I...
23 September 2019 6,075 7 View
I've used glm with backwards elimination to assess importance and interactions of multiple factors (independent variables) in a research in the field of architectural engineering. The independent...
09 September 2019 2,553 1 View