Can I ask, why Streptomyces sp. is very sensitive and easily contaminated even when doing glycerol stock? After I activate back my stock, it was found contaminated after 1 day. I have sterilize everything and doing it aseptically but still contaminated. Can someone give me some suggestion or maybe the reason why is this happen? By the way, I prepared 20% v/v for the glycerol solution.
Hello,
are you sure that it is contaminated? Some streptomycetes can be pleomorphic. They also have their complex developmental cycle, so you can see some variability in growth. How does this contamination look like under a microscope? Try streaking this contamination to another plate and see what happens.
There are also several other options I would think of:
1. You may have contaminated the glycerol stock. Concentrated glycerol will not be contaminated, although its handling may cause problems.
2. Have you checked your pipettes? Maybe someone else was handling cultures using unfiltered tips?
3. Is your autoclave working properly?
Best of luck!
The greatest difficulty in handling Streptomyces is contamination. Doubling time for Streptomyces requires 4 - 6 hours whereas many other common contaminants such as E.coli and Bacillus species have relatively shorter doubling times. Therefore, use of sterile conditions during manipulation of Streptomyces strains is highly recommended. Contamination predominantly occurs during tthe early growth phase.
- Thank you Adrian Augustyniak and Sylvester Peter Antai for your explanation
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