How to apply fuzzy K-means with spatial constraints in R?

Hello. I am looking for a solution in R to apply spatially-constrained clustering using fuzzy K-means (fuzzy c-means). I came across the spatialClust package, and was wondering whether anyone may...

30 October 2019 4,701 2 View

Does drug trafficking provide social mobility in unequal societies?

I have been reviewing literature on access to income in cities. I have found studies on labour income, subsidies, credit, remittances, informal economy and non-paid work. There are theoretical and...

09 October 2018 8,142 3 View

Are there studies showing a particular cultural relevance of private vehicles in Australia and the United States, compared to other rich countries?

Australia and the United States are among the countries with highest motorisation rates in the world. As part of my research, a country-level panel data model to analyse household transport...

06 July 2018 1,720 1 View

Formalin fixation on cryo-fresh-sections (fresh frozen) in order to use histochemistry method that only works on fixated tissues, is it possible?

I have cryo-sections on superfrost slides that were never fixed (fresh frozen) and I want to fixate it in order to use histochemistry method that only works on fixated tissues (black-gold stain...

06 July 2018 3,302 8 View

Tips for using a glass tenbroeck tissue grinder?

Hi everyone. I am grinding some brain tissues using a Tenbroeck tissue grinder. The grinder I am using is 7 mL. All of the documentation I have read online states that the grinding should be...

13 December 2017 1,831 2 View

How can I recover 3D cultures from Matrigel for a phospho-Western Blot?

I am studying vasculogenic potential of cancer stem cells, and so I culture stem cell spheres on Matrigel. After several days, they form 3D tube structures into the gel. Inhibition of my protein...

28 November 2017 8,902 8 View

How do I minimize oxygen flux when performing routine cell culture tasks on hypoxic cultures?

I am beginning to perform several experiments using cancer cells in a hypoxic environment. Our core facility has a hypoxic incubator that uses nitrogen to displace oxygen down to a minimum of 1%...

18 October 2017 6,433 3 View

Anyone have problems with PCR of LoxP inserts?

09 June 2016 8,320 7 View

Any advice on cloning a 2A sequence between 2 genes without restriction enzymes?

10 November 2015 6,691 2 View

Can I consider tonB dependent receptor as porin?

I read once in an article that tonB can be considered as gated porin, but other places doesnt agree with that...

05 September 2015 8,557 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View