We observed uneven signal intensity from the same sample loaded on the same gel
Lane 1-3: Oligo in buffer
Lane 4: Blank matrix (protein);
Lane 5-12: Oligo in matrix (protein) (same sample, mixed well before loading).
The signal appears significantly weaker in the middle lanes and stronger on the side.
Could anyone be able to provide any insight into what might cause this the uneven signal distribution?
The condition we are using as following:
Pre-cast 5% TBE 12 well Mini Gel
Running buffer: 1X TBE buffer
Loading volume: 15 µL
Voltage: 100 V
Current: default (17~36 mA during EMSA run)
Duration: 50 minutes
Gel Staining: 50 mL pf 1X SYBR Gold in 1X TBE Buffer for 10 minutes on a rocker set at the lowest speed.
Mengyi Gu
Non-denaturing TBE gels are aqueous quantum materials. In them, quantum fluctuations of water (0.3 nm) formed due to the nuclear quantum effect compete with thermal (classical) fluctuations or cavities of water in which acrylamide and DNA molecules are located. The interaction of the electromagnetic field of electrophoresis with such a fluctuating system creates an uneven picture during electrophoresis. A simpler aqueous quantum material is described in articles using surfactant micelles as an example.
Article Relationship between Classical and Quantum Mechanics in Mice...
Preprint Nuclear quantum effect in aqueous micellar surfactant solutions
Preprint Supplementary information for Nuclear quantum effect in aque...
The process is presented in the ideo.
Cover Page Quantumfluctuations-01 (4)
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