Hello all,

Recently I had WGS service with NOVOGENE including the bioinformatic analysis. I have told them the purpose of the WGS was to find the genetic variants between the WT and transgenic (TG) mice on Balb/c background in our lab. I sent them the genomic DNA of WT and TG. Their bioinformatic team compared my WT and TG to online Balb/c genomic data from ensembl and provided me the dataset of variants (long lists) in WT and TG separately. They have filtered the data for indel and snp files by remove the identic variants upon my request.

I ask them to filter the CNV (1215 rows) and SV (7985 rows) files and locate the transgene, they want to charge me $425 for filtering and $350 for transgene location. I think I should not be charged and they gave me 50% discount after I argued.

Could some bioinformaticians here to tell me whether I should pay them or they should do it for me free?

Appreciate any comment.