Polymerase Chain Reaction

In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short... | Contact experts in Polymerase Chain Reaction to get answers

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Questions related to Polymerase Chain Reaction

When PCR product is being used as template, PCR amplification is not happening. What might be the reason?

I have been trying to amplify one of the gene by PCR and I have optimised the conditions for the same and have got an amplicon. To confirm if the amplicon is my desired gene, I am using PCR...

28 October 2023 5,702 18 View

Bacterial metabolic profile?

Hi. I have a bacteria that has antagonistic properties. I would like to find out what biochemical compounds are responsible. Are there companies that do this? Maybe someone knows the pricing?

28 October 2023 8,476 7 View

How does SYBR green fluorescence work?

Dear scientific community, I was trying to understand how SYBR green fluorescent signal chnages when it binds to the DNA? Does binding to the DNA make any conformational change to it that causes...

24 October 2023 4,646 5 View

Is there a human version of BME/matrigel?

It just came cross my mind - if matrigel is derived from mice, would there be an effect on the human organoids that we are culturing/generating? If so, how can we measure the changes? is there a...

23 October 2023 413 4 View

How long can I keep my prepared sample in a fridge before adding phenol/chloroform and subsequently going ahead to extract DNA from it?

I am currently in the process of extracting DNA from microalgae using the CTAB extraction procedure. I have completed the preliminary steps but had to pause the procedure after adding my CTAB...

18 October 2023 3,311 0 View

Why is my Agarose Gel Electrophoresis always show pulling under LED Light?

My biology lab experiments for DNA isolation and screening always shows pulling and smearing under the LED light. Me and my partner were pipetting very carefully not to disrupt the well, but the...

18 October 2023 319 10 View

Is there a reference genome for RNA sequencing in Tetrahymena?

Hi! I am doing total RNA sequencing with a Nanopore sequencer to look at gene expression in Tetrahymena when mitosis is inhibited vs. normal cell division. Does anyone know if there's a reference...

17 October 2023 5,687 0 View

Why must fungal mycelium be isolated on agar before DNA extraction for PCR?

I will be collecting Armillaria spp. field samples for identification to species level using PCR. These field samples will be rhizomorphs, mycelium lifted from infected trees and in some cases...

16 October 2023 8,220 4 View

Seeking for research training in bioinformatics?

I'm on the lookout for remote bioinformatics and computational biology opportunities where I can actively contribute to research projects. Compensation is not a priority for me; my main focus is...

15 October 2023 3,706 4 View

How to measure very thick DNA band on electrophoresis?

Hi, I'm a 2nd year student in biomedical science and currently writing a lab report for which I need to measure DNA band sizes on electrophoresis. The lane for PCR amplified DNA has a very thick...

14 October 2023 7,338 8 View

Any experience in an automation of ELISA test?

It is an automated sandwich ELISA. The manual performance worked excellently. However, the automation with a pipetting robot has an issue. I diluted my sample 2, 4, and 8-fold in a serial. The 2,...

12 October 2023 3,965 0 View

Can anyone explain me difference between Taqman and SYBR green assay?

I am working on miRNA and I am advised to used SYBR green but I have seen people using Taqman, so I am confused. It will really help

12 October 2023 2,259 2 View

Which cheap FACS kit to use to isolate CD8 T cells?

Please recommend cheap and economical kits for CD8 T cells isolation using FACS, Chinese preferably.

11 October 2023 5,028 0 View

Why I do not get single colonies of Agrobacterium after electroporation? is it normal?

I did transform electrocompetent agrobacterium cells, I did not get single colonies instead I got the bacteria growing over all the solid media surface. I did select with the antibiotics. Is it...

10 October 2023 9,073 1 View

What can cause bands in gel electrophoresis not to move?

There are bright bands amplified during PCR. They are just hanging on the wells instead of moving downwards. What could be the cause and possible solution?

06 October 2023 2,976 13 View

Genomic dna electrophoresis gel contamination?

Hello, I am performing DNA extraction from mosquitoes with an in-house protocol. During the genomic DNA electrophoresis gel test, I noticed two distinct bands. I'm curious if anyone knows the...

05 October 2023 7,716 0 View

Can anyone help me identifying this organism that formed orange coloured scum layers on lakes and low running rivers in summer?

In September this year, I noticed an orange/green coloured scum layer in a slow-running river (see pictures) close to my hometown (Deinze, Belgium) containing green and orange clusters, as can be...

05 October 2023 9,462 1 View

What are the effects of contaminating denaturants from denatured ethanol on PCR?

Dear all, has anybody some experience with using denatured ethanol in nucleic acid extraction (instead of molecular grade ethanol)? I cannot find any data/description about possible inhibitory...

04 October 2023 6,313 2 View

How to remove background binding in Aptamer based ELISA?

I am using Thermo maxisorp 96 well immuno plates (https://www.thermofisher.com/order/catalog/product/437111) to attach whole virus particle. and then after incubation with FAM labelled aptamers, I...

04 October 2023 1,386 0 View

Why my stool DNA samples have A260/280 > 2?

Hello, I am extracting human DNA from stool samples using Quiagen´s QIAamp Fast DNA Stool Mini Kit. The DNA yield seems to be fine, but the A260/280 ratio is above 2.0 (2.1-2.2). What might be the...

29 September 2023 9,141 1 View

How to remove stop codon from a full mitogenome sequence?

Hi. This is my first time working with a full mitochondrial genome. This sequence was sequenced using Illumina and its consensus was produced using Geneious prime. My questions are: 1) After...

27 September 2023 7,629 3 View

Could someone recommend a relatively accurate method for analyzing DNA methylation levels at a given locus?

I am looking for a precise method to determine the level of DNA methylation at a given locus. I am working on material from cows and I wanted to adopt the Methylation-Sensitive High-Resolution...

26 September 2023 4,655 1 View

Please i need a detailed laboratory procedures for isolation of preadipocyte from the longissimus muscle of rabbit?

If a video is available, it will assist me more. And the adipocyte tissue culture procedures

26 September 2023 4,611 0 View

Is there any publicly available dataset for abiotic stress severity classification?

I am struggling to get any dataset required for abiotic stress severity classification. Can you please suggest?

23 September 2023 5,867 0 View

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