Given the 2018 IUPAC recommendations, to what extent is it valid and relevant to classify molecular diagnosis and molecular characterisation within the domain of chemistry, rather than exclusively under biology or medicine?
I'm planning on activating 5 million PBMCs with CD3/CD28 at the same time applying a treatment, then incubate for 72 hours. Then I seperate cell subtypes using antibody sequencial seperation...
01 July 2024 8,892 3 View
I am trying to use CatBoost method (a gradient boosting method) for flood susceptibility mapping. I have seen that many metaheuristic algorithms were used to optimize Support vector machine,...
11 December 2022 993 6 View
I tried to transform Physcomitrella patens with Em-GUS cassette by using PEG. After two round selection with 15µg/ml final concentration of hygromycin, I had few transformed lines. However, When I...
03 January 2016 2,253 3 View
We are currently developing some guidelines.
29 May 2014 6,654 5 View
I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...
08 August 2024 8,329 0 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
I created a file with my outgroup and ingroup species using Beauti, ran it in BEAST, viewed it in Tracer, and then used TreeAnnotator to create a file that I imported into RASP. Could someone...
28 July 2024 2,979 1 View
PeptiCloud (www.pepticloud.com) is a bioinformatic platform that allows researchers to organize and share their data for their projects as well as collaborate with others in one place. Through...
28 July 2024 4,762 2 View
Do you know of any online international conferences that offer free discussions? I am looking for examples in the field of molecular ecology and DIY biology.
28 July 2024 6,501 0 View
Dear Siesta Users, I installed Siesta 5.0.1 and I want to use Grimme's D3 correction. According to the 5.0.1 manual, I could use a few parameters to include the D3 correction in the...
27 July 2024 5,748 0 View
It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...
25 July 2024 8,738 0 View
Hello, We found three packages of Illustra™ MicroSpin™ G-25 columns in the cabinet of an unused lab. They are very old but have never been opened. I have never used this kit before, and I couldn't...
25 July 2024 4,927 3 View