Is there any database or website from where I can get a list of FDA approved drugs for treatment of certain urinary tract infection?

I want to know all prescription drugs which are FDA approved compounds for infection like Urinary Tract Infections along with their causative agents. Can you guide me about any resource, database...

17 December 2020 4,852 3 View

Problem downloading Sentinel-2 Offline data ?

I am trying to download Sentinel-2 data of year 2018 over Hong Kong and the Pearl River Estuary. But as 2018 data is offline I am neither able to download it using python batch processing script...

05 April 2020 7,852 6 View

How to calculate multiplicity of infection(MOI)?

04 March 2020 4,208 3 View

Can anyone help me for the extraction of DNA from human saliva?

I have performed this experiment several times but failed.The main problem is that its UV spectra has a lot of distortion and its peak is not on 260nm. The DNA which i extracted has 1.5...

03 August 2019 4,614 3 View

How to convert analog signal to digital data using python?

How to convert analog signal to digital data using python code?

26 May 2019 1,498 3 View

How to convert 2D images to 3D?

How to convert 2D images to 3D using python for medical images?

20 May 2019 722 2 View

How to convert 2D images to 3D?

How to convert 2D images to 3D using python for medical images?

04 May 2019 755 2 View

Can we predict earthquakes using Deep Learning in a real time?

Can we predict earthquakes using Deep Learning in a real time? I use python but the analysis process takes long time.

04 May 2019 4,627 4 View

How can we stain liposomes with rhodamine for fluorescent microscopy?

I want to stain liposome with rhodamine. For this I need protocol.

20 April 2019 7,138 1 View

What should be the sonication time to disperse Magnetite nanoparticles for SEM?

I am using probe ultrasonicator to disperse MNPs in ethanol for SEM.

25 January 2019 2,091 8 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View

How T cells recognise non-peptide antigens?

This might sound very basic question, but please share with me an explanation of how the T cell is activated when the antigen is not a peptide? What happens? And what are the molecules involved in...

21 February 2021 564 3 View

Different antibodies show signal at same MW in SDS-PAGE, what is wrong?

16 February 2021 2,857 9 View

Is there such a thing as Igg specific MHC class II epitopes or do all non-self MHC class II epitopes trigger antibody production of all subtypes?

I know there are bioinformatic tools that allow epitope prediction to determine if a certain peptide would trigger an immune response. I also read that for allergen diagnoses IgE of sensitised...

09 February 2021 4,173 3 View

Will it be possible to get IC:50 if we target cancer cell line with antibody drug conjugate (ADC) that have 50% less antigen positive cells ?

Is it possible to attain IC50 if my target cells have less than 50% antigen expression ? For example: Cancer cell line XXX is showing 35% of expression of cell surface antigen YYYY, if i...

04 February 2021 2,711 3 View

Can I heat my ELISA at 37C?

Hello, I am conducting a sandwich-type ELISA. It is to see if small molecules can interfere with a protein-protein interaction. As this interaction would occur in the human body, may I heat...

31 January 2021 4,271 3 View

How to characterize epitopes within a peptide sequence?

Greetings In a theoretical perspective, I want to use a human-derived peptide as a plausible antigen but the problem associated with tolerance hampers my approach. Then I thought why not denature...

20 January 2021 2,201 1 View

How can I do antigen retrieval for proliferative markers (MCM2, Ki67 etc) While preserving a sensitive endogenous reporter?

I am trying to do antigen retrieval on mouse brain sections to stain for proliferative markers like MCM2, Ki67, PCNA etc on tissue that has mCherry conjugated to a transcription factor of interest...

18 January 2021 1,023 3 View

Can anyone walk me through IHC/IF Staining of Cryosectioned Brain Tissues?

I'm looking to do some IHC/IF staining of X. laevis brains that have been frozen in OCT media for cryosectioning. I understand how to do the cryosectioning, but I am hoping for help/protocols that...

14 January 2021 9,752 2 View