Hello!
I really hope anyone can help me out.
For my master thesis I am comparing cancer cell clones and their reaction to certain targeted inhibitors.
Now I would like to perform a two way ANOVA test for my data and I am struggling with the set up.
The null hypothesis is that the drug has no cytotoxic effect on the cells.
Here is the design of my data. I work with triplicates. The cells are seeded in a 96 well plate with the following set up.
Concentration: Clone Response (SRB intensity in %)
Control Control response
C1 R1
C2 R2
C3 R3
C4 R4
C5 R5
Since the two-way ANOVA test compares two data sets my first dataset is the one with the responses, correct?
Do I create a second data set where I simply put in all the responses for every concentration as 100%, artificially creating the set up that the drug has no effect?
Hello Erika,
First, I'm inferring from your query that concentration level represents one IV. But, I'm unclear as to what the second IV might be. Is it drug? Replication (e.g., three trials of each concentration)? Something else?
Second, I'm not clear as to why you want to use GraphPad (perhaps, other than prior familiarity). The one time I looked at the user manual for this to address someone else's RG post, I was amazed at how non-standard one's data set-up needs to be for multi-factor designs (esp. mixed models). You might consider the freely available Jamovi package as a better alternative.
Good luck with your work.
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