Chemical compounds that occur naturally in plants | Contact experts in Phytochemistry to get answers
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Questions related to Phytochemistry
I prepared the powder of methanolic extract than I want to desolved for testing this extract on the fungus . How to desolved it whith water or with in other solvent .???
24 January 2017 5,472 5 View
I was trying to find the zone of inhibition for Lawsonia inermis, so I had to stack pieces of filter paper for different concentration (more no of filter papers stacked- higher concentration)....
22 January 2017 7,561 5 View
I am trying to identify the volatile emission spectrum from A. thaliana and L. sativa using SPME of their headspace (static) with GC-MS. I am using an Agilent HP-PONA column, and a GC program with...
19 January 2017 4,573 12 View
Actually, there is a range of articles about quantification of phenolics/antioxidant activity and methanol is widely used for this purpose because of its good polarity and performance to allow...
17 January 2017 7,379 2 View
I work on an ion chromatography system for the separation of anionic species in wine. Since I changed the Guard and Separation Colums, backpressure increased from 1500 to 1900 psi at my working...
12 December 2016 5,834 5 View
I follow Ruch et al 1989 method for h202 scavenging assay but instead of decrease in absorbance I am getting increase in absorbance. I am using phosphate buffer as blank, h202(40mM)+phosphate...
12 December 2016 8,483 4 View
08 December 2016 5,411 4 View
Hello, This week I measured the DPPH scavenging activity (%) and the antioxidant activity expressed in VCAE in 2 varieties (red and yellow) of onions. (Find protocol attached). Unfortunately the...
11 November 2016 5,214 3 View
Both 1H and C13 NMR values of my sample can be assigned to a known molecule except at one place. From reference values and theoretical point of view, a methine proton (CH) near to a methylene...
11 November 2016 2,861 5 View
I was estimating total sugar in rice leaf and stem at the vegetative stage. I got more sugar in leaf than the stem. Is this correct? If yes then why? Example- Leaf-10.32 mg/g FW and Stem-3.73...
11 November 2016 4,318 3 View
08 November 2016 8,098 5 View
I used bates method for proline estimation. I used 0.5 gm dried leaf sample (wheat) and homogenized in 10 ml of sulphosalicylic acid. From that stock solution 0.1 ml sample was taken and went...
17 October 2016 5,789 5 View
10 October 2016 3,865 5 View
I want a protocol to determine amylose and amylopectin composition in rice..
21 September 2016 5,459 5 View
Phytoalexins are only one component of the complex mechanisms for disease resistance in plants caused by fungi and bacteria (Kuc, 1995). Easy to find the antimicrobial mechanism of phytoalexins on...
09 September 2016 3,330 3 View
I came across the call for proposal for The Finland ministry of Foreign affairs known as HEI ICI Programme 2016–2018 with deadline of October 5th. The collaborator should a university from...
09 September 2016 5,453 4 View
I was using Bradfords reagent for protein quantification in microplate reader. The problem I faced was I got variations in house keeping gene. The pity is that some sample even did not...
09 September 2016 6,925 1 View
I am researching various cannabinoid delivery systems. I have a viscous ethanol cannabinoid extract that I wish to turn into a powder. I need it to be a powder preferably one that is water soluble...
09 September 2016 5,147 6 View
Please provide reference/ protocol for the extraction of metabolites from the mycelia of penicillium after the filtration step ?
09 September 2016 4,523 3 View
we observed that the neem extract decreased total RBC when increased WBC in different neem extract concentration exposed fish.
09 September 2016 5,277 2 View
Please mention the concentration as well for the study of heavy metal.
09 September 2016 8,530 2 View
09 September 2016 8,972 5 View
07 September 2016 3,363 1 View
06 September 2016 1,233 3 View