i am doing research on medicinal plant. when i estimate total alkaloid content face a lot of problem and failure to get result. so i request any body share the protocol for alkaloid estimation
Have you tried a selective extraction with acid-base extraction ? I think you can weigh it first and then use a reactive like dragendorff's reactive to test whether are alkaloids in the partition.
Alkaloids are basic compounds, so you can determine them by titrimetry in aqueous or not aqueous media. In the first case you should use sulphuric or clorhidric acid, in the second case use perchloric acid.