i need to remove chlorophyll from leaf material so that chlorophyll content donot hamper the isolation of pure compound. Does ethanol gradient method will effect the extraction procedure in any way?
Usually chloroform or dicholoromethanol will remove the pigmented compound . Before ethanol extraction the leaf material should be extracted with chloroform or dichloromethanol
As per Kalaivani or Walsed, the method may work but it all depends upon the dipole moment of the compounds you are looking for. Chloroform or acetone are polar solvents and they might remove some essential compounds along with chlorophyll.