The compounds are metal complexes wherein a Nickel compound has shown effective antimicrobial activity against MRSA strain, Vanadium compound has shown antidiabetic activity and copper compound, a very good anticancer activity.
I need to know if performing DPPH assay for determining the antioxidant activity add on or give an extra proof for the above said activities?
DPPH test is widely performed to assay anti oxidant/free radical scavenging activity of compounds. Free radicals are generated through oxidative stress and attributes to the tissue damage and promotes end organ damage like liver fibrosis or ageing process. These also induce mutation which can lead to cancer. Since this test has a vast array of significance, it cannot be a confirmation test for the prognosis of cancer. Rather you can go for cell line assay. Please follow the link.
https://www.ncbi.nlm.nih.gov/pubmed/23963912
Free radicals are responsible for several deared diseases including some types of cancer, diabetes, atherosclerosis, neurodegenerative diseases and ageing. Antioxidants have primarily preventive role. Your compounds have antidiabetic, anticancer antimicrobial property. So both chances ate there. These compounds may or may not positively respond to DPPH radical scavenging test.
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