Hi

uou can use conventional PCR by selecting a specific primer for R solani or using 16SrDNA

Dear Aqleem Abbas

Pls. find the attached files

https://www.ncbi.nlm.nih.gov/pubmed/8821665

http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582002000300004

https://www.researchgate.net/publication/14383011_Identification_at_strain_level_of_Rhizoctonia_solani_AG4_isolates_by_direct_sequence_of_asymetric_PCR_products_of_the_ITS_regions

page 212 in: G.G. Khachatourians, Dilip K Arora . 2004. Fungal Genomics Elsevier, Pp. 432.SBN 0080527469, 9780080527468

http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582003000400011

https://www.researchgate.net/profile/Oscar_Salazar7/publication/14383011_Identification_at_strain_level_of_Rhizoctonia_solani_AG4_isolates_by_direct_sequence_of_asymetric_PCR_products_of_the_ITS_regions/links/0f31753b436131aa70000000.pdf

http://link.springer.com/article/10.1007/BF02221582

https://theses.lib.vt.edu/theses/available/etd-08142011-170650/unrestricted/Amaradasa_BS_D_2011.pdf

Pradeep Kumar, Vijai Kumar Gupta, Ajay Kumar Tiwari, Madhu Kamle. 2016. Current Trends in Plant Disease Diagnostics and Management Practices Springer,Pp.469. ISBN 3319273124, 9783319273129

Hoping this will be helpful

Regards

Prof. Houda Kawas

Article Identification at strain level of Rhizoctonia solani AG4 iso...

Dear colleague

Follow this link

http://femsle.oxfordjournals.org/content/162/2/289

regards 

77

Hi Abbas

You can use ITS1 as forward and ITS4 as reverse

Dear, 

Their are may publications in this respect and yo can use one of them for identification of R.solani.

best wishes.

Thank you all of the researchers, who contributed to my questions. I will follow your suggestions. 

You most welcome

Houda

You are welcome