Why are PCR products non-methylated ?

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Charvi Nangia

True..but the DNA methyl transferases would methylate them soon...isn't it? How long do they stay unmethylated?

Gertrudis Rojas

In vitro synthesized DNA strands (that have never been inside a cell) remain non-methylated. Template strands coming from E.coli remain methylated.These are the bases for DpnI discrimination.

Amruta Dhawale

So is it necessary to do DpnI digestion for every PCR products which we want to transform or use in ligation experiments ?