Can we use quantitative gene expression levels without reference dye in qPCR MASTER MIX?

Hello I had a small question regarding the reference dye that incorporated in qPCR master mix of 2x Syber green or Taq Man mix. Generally these reference dyes such as ROX is incorporated for the...

07 August 2018 990 0 View

Whether to maintain RNA or Cdna concentration constant with control to measure gene expression level in Real time qPCR.?

Hello I am working on the real time PCR for quantifying gene expression levels. Since the conversion rate of RNA to cDNA depends on reverse transcriptase efficiency which things to be consider for...

11 December 2016 4,413 8 View

Has anyone worked on measuring autophagy induced genes by realtime PCR?

31 December 2014 3,284 9 View

Why deep freeze will generate more heat during the initial installation?

31 December 2014 9,471 0 View

Will it be possible to see the mutational changes in the dengue viral NS 1 construct by site directed mutagenesis?

11 December 2014 4,774 3 View

Has anyone found the expression of a protein of interest in the mammalian cell lysate by ELISA?

I want to see the protein expression in which the corresponding gene is cloned in a mammalian vector without a secretory signal. I want to see its protein expression in ELISA by using the lysate...

10 November 2014 6,844 5 View

Will the background caused by mammalian cell lysate matter in ELISA?

Can you tell me whether the background caused by cell lysate matters in giving the signal? wil it be okay if I take lysate cell supernatant after short centrifugation?

10 November 2014 1,354 3 View

Has anyone used autophagy dye from Enzo Life Sciences?

10 November 2014 8,199 1 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View