How to remove the species data from rda data frame in R (only remain the sites/samples point)?

Dear everyone, When I using the rda function in r, I met this question. As you can see in the figure, there are a lot of deep blue points which refers to the species. But I would like remain...

15 February 2021 8,982 2 View

Whether L version and D version peptidesshould have identical NMR, especially in the state of self-assembly?

Whether L version and D version peptides should have identical NMR, especially in the state of self-assembly have to have identical NMR? How to use HRMS to confirm L version or D version...

01 February 2021 6,756 4 View

What are possible factors influencing the isotope-based hydrograph separation in river confluences?

When performing hydrograph separation (EMMA method) with isotope tracers in river confluences (natural large basin), we found that the contribution of each end member sometimes exceeds the...

20 January 2021 8,403 5 View

Can you have pressure inlet and velocity inlet at the same time?

I was reading a reference (Reitz-A Photographic Study of Flash Boiling Atomization). In his experiment, his boundary condition is delta p and constant velocity. The liquid injection pressure was...

18 January 2021 842 9 View

How to determine additional cycle numbers using qPCR in ATAC-Seq?

I am doing ATAC-Seq with OMNI-ATAC-Seq protocol (Kaestner Lab 2019). I did PCR amplification for 5 cycles of my library. Now I use 5ul of the PCR product to do a qPCR to determine how many...

12 November 2020 3,678 1 View

How to visualize influenza PR8 plaques while the infection is successful?

Hi, everyone, I am optimizing my protocol of influenza PR8 plaque assay. I am following the classic protocol. But I ran into problems when I am trying to visualize plaques at the high virus...

25 August 2020 2,017 1 View

How to extract absolute refractive indices of an optical fiber from an refractive index difference plot?

10 May 2020 2,402 13 View

Do you think current guidance for COVID-19 prevention is correct?

CDC provided guidance to the public "Wash hand, disinfect surfaces, cover sneezing" ever since the outbreak of COVID-19. I get a strong sense this guidance is for contact transmitted disease...

01 April 2020 8,279 1 View

Where is the TSS (transcription start site) following CAG promoter?

The commonly used CAG promoter is usually labeled as three parts, take pCAGGS-mCherry (https://www.addgene.org/41583/) for example. CMV enhancer + chicken beta-actin promoter + chimeric...

13 February 2020 7,125 2 View

Google scholar inflates citation count?

Google scholar included under "Cited by" a few papers that did NOT cite my papers or book chapters. This inflates citation counts of some of my publications. How can this be corrected?

29 November 2019 2,469 4 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Why does two different bioinformatics tools for the same proteins show different subcellular localizations?

During a computational analysis, I found that for a protein of a plant, two different subcellular localizations are seen using CELLO and WoLF PSORT. For example, in CELLO, it identified the...

01 March 2021 6,729 2 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View

Can we remove the fmoc group after peptide cleavage?

I have synthesized the peptide using Fmoc based solid phase peptide synthesis. Also I have cleaved the peptide from the resin and lyophilized it, but later realized that, I forgot to deprotect the...

21 February 2021 4,537 3 View

Antibody amino acid sequence database to simulation?

Hello, I need your help. I am looking for a database of antibody amino acid sequences for later simulation in a program in order to analyze their structure. The antibodies I need to test...

18 February 2021 371 4 View

Is my GFAP staining in right position?

I was differentiate hESC to astrocyte. In the second week of differentiation, I stained my cell with s100beta and GFAP. I expected that the staining site of GFAP would come from the cytoplasm,...

15 February 2021 8,982 1 View

Does the property of GFP affect a fusion protein's localization?

Hello, I am planning to insert GOI between CaMV 35 promoter and mGFP in pCambia1302 to produce a fusion protein and see subcellular localization of the protein. But the vector says it's membrane...

15 February 2021 4,353 3 View

Peptide precipitation as pre-step for acid hydrolysis for amino acid analysis?

I'm currently experiencing problems during acid hydrolysis for total amino acid analysis as my first samples contain high amounts of sugar (I get too low results back). I therefore had the idea to...

14 February 2021 6,049 3 View

How can I select the best plasmid after sequencing?

I have transformed a drought-related gene of 1.7kb into pGEMt vector (E.coli DH5 alpha strain). After sequencing the plasmids (3 replicates) 4 changed amino acids are visible at different...

06 February 2021 336 3 View

Identical spectra for L- and D-peptides after they self-assemble?

Hi dear all, I need advice. I have two peptides with the same sequence but one consists of L-amino acids only and the other consists of D-amino acids only. when they self-assemble in aqueous...

03 February 2021 2,032 3 View