Who has already compared sediment cores downstream of dams with proxies of discharges time series and/or suspended sediment fluxes?

01 June 2021 0 3K Report

The objective is to assess the impact of dams on the reduction of solid transport.

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How can I improve my electrophoresis technique?

Hello. I'm making non-denaturing PAGE of DNA fragments for analysis of polymorphisms, but I'm getting wavy bands. If somebody can help me, I will be very greatful. First of all, here's my recipe...

15 March 2024 7,699 1 View

Can cy5 be fixed using formaldehyde ?

Hello I am planning to deliver a cy5-labeled drug to mice, and then check its distribution in different organs. In case I wish to preserve the tissues for later research, will fixation (using 4%...

12 November 2023 4,958 1 View

Lentiviral transduced cells loose insert?

I have infected iPSCs with a lentivirus and sorted GFP positive cells. But after just a few rounds I am finding cells without GFP in culture. Had anyone seen this? Maybe cells are segregating...

17 October 2023 1,611 1 View

SORTING of iPSCs cells?

We are trying to purify human iPSCs using lentiviral induced GFP expression. But is being really difficult to achive this. Cells are usually dying after sorting. Any experience on this? anyone...

25 September 2023 6,985 2 View

In modern shunt system what is a typical distal catheter length within the peritoneal cavity?

In the 2010 era, it was typical to provide distal catheter lengths that were 2 to 3 times the height of the cavity to allow it to slide and bend away from obstructions. The force necessary to stop...

02 September 2023 10,029 0 View

Non-parametric Kruskal Wallis instead of a two-way ANOVA?

Can I apply a non-parametric Kruskal Wallis test instead of a two-way ANOVA when assumptions of normality and heterocedasticity are not met?

11 April 2023 4,229 11 View

Why is it expected to get a high percentage (≥80) of uniquely aligned reads in RNA sequencing?

I started learning how to use Tophat tool. I used it to check RNA sequencing and I calculated 64.91% in the percentage of uniquely aligned reads. I read in article that in RNA sequencing we should...

14 November 2022 7,252 3 View

Does anyone know about transformation methods in Mycetohabitans spp?

I'm having trouble transforming my bacteria.

09 August 2022 2,357 0 View

A question regarding the ultracentrifugation method for exosomes isolation?

Hi, In the ultracentrifugation method, there are several steps in which one collects the supernatant ,discard the tube with the sediment, transfer the supernatant into a new tube for a higher RCF...

14 November 2021 182 3 View

Why do I get smaller fragments than expected after PCR in Long Range PCR?

I'm looking into amplifying a 5kb region within the ChrX (DNA input- genomicDNA). I have been trying to use several primer pairs and the best performance (in terms of amplification) were (a)...

12 May 2021 2,012 3 View

Could you recommend some articles on Urban Transportation System optimization and Innovation?

13 August 2024 2,595 3 View

How are iso-frequency contours plotted?

Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...

07 August 2024 1,937 1 View

How to correctly use the UTE and ZTE pulse sequences in Bruker's ParaVision software?

I am using a Bruker 600M solid-state NMR spectrometer with a Micro 2.5 microimaging system. The test sample is a tube of 1M LiCl aqueous solution, and the nucleus detected is 1H. I am trying to...

01 August 2024 9,227 1 View

Is the black sediment consider as sand? If not, how can I filter it out?

Hi researchers! I'm working on soil texture analysis, and the end result for sand is doubtful because there is black sediment appearing after drying, as shown in the figure. Is it considered sand?...

30 July 2024 557 2 View

What is the solubility of Iron (+3) in Bismuth Oxide?

Dear all: I am modifying Bismuth Tungstate with Hematite (Fe2O3), so I would like to know about how much Iron(+3) can form a solid solution in a Bismuth Oxide matrix. Best wishes Marco

29 July 2024 2,163 1 View

Optimal siRNA Volume for Complexation with Liposomes in a 2.5 mL Solution with 10 mg/mL Lipid Concentration?

How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?

27 July 2024 6,920 1 View

Which transport medium is best for E.coli?

I'm working on Microbiology EQA program. I have confirmed E.coli strains in my lab. Now I want to transport them to other labs at a distance of 10-12 hrs. we don't want to use ice-packs during...

23 July 2024 1,855 0 View

Prevent sedimentation and maintain the suspension of powder particles in the resin ?

Hello, I am experiencing an issue with mixing micro powder with resin in a 3D printer. The problem is that sedimentation occurs after a short period (about half an hour to an hour). Could you...

23 July 2024 1,672 0 View

Look for qualified candidates of Visiting Scholars to Southwest Jiaotong University?

We invite qualified candidates of Visiting Scholars to engage in research collaboration or deliver lectures on their research, with travel expenses covered. The visiting period is flexible,...

20 July 2024 1,165 1 View

How do I prepare a working stock solution of 14C sodium bicarbonate to get a final activity of 3uCi?

I need to make a stock solution for radioisotopic incubations of freshwater sediments to study dark primary productivity but I have some confusion as to how I calculate the concentration of 14C...

15 July 2024 5,898 0 View