I am planning to use cobalt chloride hexahydrate to incude HIF-1a in my cells which grows in RPMI+FBS media. Will cobalt chloride cause precipitation of proteins in media during incubation?
The short answer is No. At the concentration (100 micrmolar) of CoCl2 that is needed to induce HIF-1alpha (see our study in link), it does not precipitate proteins in the culture medium.
http://www.oncotarget.com/index.php?journal=oncotarget&page=article&op=view&path[]=8594&pubmed-linkout=1
hello all, thanks for the responses. I have never used cobalt chloride before and i have to use it now. i am considering the safety measures. I do understand the main risk is while weighing and dissolving it. I am planning to buy Cobalt chloride 0.1 M solution from sigma aldrich. i will off course be treating the cells with it inside the hood, i guess this is sufficient safety measure. but i wanted to ask how to handle the media post treatment and cell/cell lysate post treatment. Thanks in advance.
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