Can anyone tell what it would be like if I use goat blood instead of sheep blood to make blood agar for bacterial colonial growth?

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Where to download DEM from 2018?

I am currently working on validating land subsidence results obtained using SBAS InSAR. Could anyone suggest reliable sources or repositories where I can download a historical DEM from 2018?...

31 July 2024 9,757 3 View

What change would occur in physics if the three different sizes of the proton and the two sizes of the deuteron accepted as new physical constants?

For people today, the enigma of the size of the proton still remains enigmatic. However, being myself a researcher in new physical theory which makes it possible to extend the different standard...

28 July 2024 7,823 1 View

Can light itself have a temporal property? I mean, does light capable to dilate time if conditions require it?

About time dilation. We remember the descriptive diagram which includes, for example, the train which moves at a speed "v". The two mirrors installed in the train between which a beam of light...

03 July 2024 4,332 1 View

Do you think that the mcc energy must result from the sum of two energies related to wave-particle duality?

29 June 2024 1,915 0 View

Human health risk assessment of ambient air pollutants, how to calculate hazard quotient?

I have calculated CDI (inhalation) of Ozone, CO, SO2, NO2, NO, and TSP (total suspended particulate matter) . However I cannot find RfC (Reference concentration) or even NOAEL of any of these...

27 June 2024 4,488 0 View

What is the connection between the "proper energy" of a particle, the "limit energy" of a particle, and the "intrinsic energy" of a particle?

The “intrinsic energy” of a particle is well known, it is mcc so what about of the “self energy” of a particle if one want to call it so and the “limit energy” of a particle if one want to call it...

24 June 2024 3,366 3 View

Thesis For BBA Students?

Hi, My name is Muhammad Afnan Tariq. I'm 6th semester student at university in pakistan and currently enrolled in BBA Program. I want to write a thesis as fyp. I have interest in Marketing and...

23 June 2024 9,160 3 View

Is the Moderator variable different from the mediating variable?

11 June 2024 7,855 3 View

Do you think the charge is the ultimate origin of the mass of a particle it provide to the particle via a very specific spatio-temporal process?

07 June 2024 8,884 4 View

In a water balance computation, Which is better to use GETFLOWS or FEFLOW Model?

I mean in surface, evapotranspiration and groundwater interaction

04 June 2024 9,478 0 View

Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?

29 July 2024 10,023 2 View

Pink bacterial colonies?

I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...

22 July 2024 5,953 6 View

Should all lactic acid bacteria be catalase negative? should all probiotic bacteria be catalase negative?

I am working on probiotic bacteria for fish, and I am trying to isolate probiotic bacteria like lactic acid bacteria or other probiotics from the gastrointestinal tract of fish using different...

12 July 2024 3,756 4 View

Can we change blood agar in the mCIM and eCIM protocols?

In the modified carbapenem inactivation method (mCIM) and EDTA-carbapenem inactivation method (eCIM) protocols, can we replace blood agar with another culture medium?

10 July 2024 6,352 0 View

Arabidopsis contamination in Hydroponic Culture ?

Hi I have a real problem growing Arabidopsis in hydroponic culture .I am using the bvoxes from Araponics and a Binder growth chamber with short day conditions and everything accurate .the seeds...

25 June 2024 9,151 6 View

Why I am not getting colony after transformation in Dh5alpha?

I am trying to do gibson assembly cloning. I have two fragments for gibson assembly cloning (plasmid backbone and insert). After gibson assembly reaction I have transformed this in E. coli....

19 June 2024 6,404 4 View

Pseudomonas aeruginosa colonies are too transparent: any tips on counting?

Hi everyone. I need to do a lot of colony counting tomorrow and when I was doing it today I’ve noticed that most of the colonies are too transparent - I have to shine bright light on them all the...

12 June 2024 711 3 View

Media Preparation protocol for Bifidobacteria Agar and Broth?

Hello everyone! I have been trying to develop a protocol for my thesis research which includes culturing and storing Bifidobacteria. I have come across many articles and studies related to the...

10 June 2024 4,152 1 View

I am working on antimicrobial effect of CBD (Cannabidiol), how should I prepare the stock which dissolve in Muller Hinton agar?

I am working on antimicrobial effect of CBD (Cannabidiol). As it is insoluble in water, I prepared a stock of 500 mg/ml (in DMSO). Now I have two problems: 1- I can not dissolve more than 500 mg...

10 June 2024 9,564 6 View

Which is the best media for revival of Pseudomonas aeruginosa?

Hi I am trying to revive Pseudomonas aeruginosa from frozen glycerol stock and also from cryobeads. But there was no bacterial growth. I used nutrient agar, tryptone soy agar to streak on. Also I...

09 June 2024 6,651 9 View