What is the effect of differentating 3T3-L1 preadipocytes in differentiation cocktail for 3 versus 7 days?

12 December 2017 1 774 Report

I noticed that some protocols use differentiation media for 2-3 days while others go for 5-7 days before changing to maintenance media. Why is that?

Is an adipocyte grown in DM 3 days + MM 4 days less mature than an adipocyte grown in DM 7 days? Also, does maintenance media purely "maintain" the current adipogenic state of the adipocyte, or does it still allow further differentiation?

Emmani Nascimento

The components present in the differentiation medium initiate your precursor cells to differentiate (eg PPARg agonist, insulin and IBMX). Your cells respond to this stimulus or they don't. Adding this stimulus for 7 days will not change/enhance the initial impulse for differentiation.

I've attached a tech bulletin from ATCC on 3T3-L1 differentiation with some wet lab pointers for adipocyte differentiation.

https://www.atcc.org/~/media/PDFs/Technical%20Bulletins/tb09.ashx

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