Hello everyone,
I have been using KRP as a media for PMNs during ROS assays (luminescence based detection of ROS generation upon infection of PMNs with Strep Pneumo).
I couldn't find in the literature why KRP is the buffer of choice for such experiment.
Does anyone knows why KRP is used instead of let's say HBSS-?
Thank you
If there's no previous literature why use it.... or can trying with this media contain KRB buffer and compare your results with standard media
probably this will be selective media for Strep. Pneumoniae
As a buffer, it has a basic function to maintain the pH and osmotic balance in the medium and to provide the cells with water and essential inorganic ions, as they are alive when you are doing ROS assay. Other or additional reason could be that in this phosphate buffer, the luminescence overall is better from the reagents compared to other buffers. Has someone in the lab done some pre-liminary experiments to compare the luminescence efficiencies among st different buffer systems? I guess you can use HBSS buffer if it does not contain sugar source (which it has; glucose).
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