Hello all,
I wish to perform a luciferase luminescent measurement of ATP in E. coli.
I have acquired a kit that requires less than 1 µM of KCl (and other monovalent metal salts). Also divalent metals must be in the ~3 mM range, and EDTA inhibits the reaction.
Normally, I would perform serial dilutions of the cells in PBS, but this raises the concentration of salts too much.
I thought about using Tris-HCl 0.01 M, but the only protocols i found are for cell lysis. I want to lyse the cells but not immediately.
Can i use Tris 0.01 M at 7.4 pH for the serial dilution of my cells without them suffering stress?