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Particularly, I want to know the cultured cell ATP assay Protocol, and data analysis (I am working on GBM cell line). Any prompt reply would be highly appreciated Thank you,

09 October 2018 2,182 0 View

What is the required concentration of all three (Packaging, Envelop, and Transfer) vectors in Lentivirus Packaging?

I need to package lentivirus to generate gene of interest stable brain tumor cell line so, I want to know what is the required concentration of packaging, envelop and transfer vectors to transfect...

31 December 2017 2,649 2 View

How to interpret JC-10 Staining results

Hello Dear Researchers does anyone have experience to interpret JC-10 staining result for Mitochondria Membrane Potential. Could someone please explain me in detail with images because yet i don't...

11 December 2016 8,266 0 View

Manual Protein Extraction Protocol?

10 November 2016 8,427 2 View

What if re-autoclave PBS twice for Cell culture use?

what if re-autoclave PBS twice for Cell culture use? it will work normal or not?

09 October 2016 1,465 0 View

What should be the minimum DMSO concentration to dissolve Temozolomide drug?

09 October 2016 2,937 3 View

Does anyone have exprerience with the situation when Insert and plasmid are not ligating?

I did the digestion of gene X and gene Y with respective enzyme and plasmid concentration and insert concentration and size everything is fine after digestion but after ligation I am not getting...

08 September 2013 8,036 2 View

Not getting desired band of inserts after digesting with respective enzymes?

I want to do subcloning of two genes (gene X and gene Y) in pcDNA 3.1+ expression vector. Gene X is having KpnI and NheI restriction site, gene Y having BamHI and NheI restriction sites....

08 September 2013 3,434 12 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

No title

28 February 2021 9,936 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

What is Flat Lens Antenna?

28 February 2021 5,433 3 View

Which one carries more value? A research paper in a Scopus Indexed conference proceedings or the same paper in a Journal without Scopus indexing?

I am looking for the suggestion among which one to pick from the above two categories for a research work.

27 February 2021 10,025 1 View