Hello all,
I wish to perform a luciferase luminescent measurement of ATP in E. coli.
I have acquired a kit that requires less than 1 µM of KCl (and other monovalent metal salts). Also divalent metals must be in the ~3 mM range, and EDTA inhibits the reaction.
Normally, I would perform serial dilutions of the cells in PBS, but this raises the concentration of salts too much.
I thought about using Tris-HCl 0.01 M, but the only protocols i found are for cell lysis. I want to lyse the cells but not immediately.
Can i use Tris 0.01 M at 7.4 pH for the serial dilution of my cells without them suffering stress?
check this links the articls maybe help you
Article Predicting Stability of DNA Duplexes in Solutions Containing...
https://www.nature.com/articles/srep08512
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