I am trying to separate the various oligomers of RNase A, and want to use the non-denaturing gels for this purpose. Can anyone suggest the type of gel that needs to be used for this purpose. I need to run the gel at pH of 5 with Sodium Acetate as my running buffer( electrolyte). My intention is not to denature the protein system by adding SDS or anything else. Thank you for your suggestion.
Try to use discontinuous polyacrylamide slab native gel electrophoresis 12 -15% without SDS .The gel must equilibrated with 150 mM sodium acetate buffer pH 5 for 20 min. then incubated in substrate solution consisting of 150 mM sodium acetate buffer pH5 2.5 mM EDTA 0.4% pure RNA for 30 min at 37C. rinse with equilbration buffer stained with 0.2% toluidine blue 0.5% acetic acid for 5 min finally destained with 0.5% acetic acid until clear bands of RNase appeared Good luck
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