How does trust influence the development of environmental projects?

Hello Scholars, I am exploring "trust" relevance in the development of socio-technical systems. What is your perception of cognitive values like trust to the energy transition/implementation of...

09 February 2021 8,133 5 View

How to increase AZ 40XT adhesion on conductive metal?

05 October 2020 2,899 3 View

What are the nuclei mean number from freeze tissue nuclei isolation protocol?

Hello, everyone! I'm going to start a project with single-cell ATAC-seq for analysis of open chromatin regions from freeze tissue samples. I'm lost about the nuclei concentration. If I made a...

19 June 2020 7,348 2 View

Can I have a list of genes from differents genomic coordinates?

Hello, I have a list of genomic coordinates from differentially methylation regions and a I need to extract from them a list of genes, and I don't want do look one region by one. Is there a R...

02 January 2020 7,058 4 View

How to properly calculate the mutational rate in bacteria?

Hello everyone, I hope y'all can answer a simple but problematic question. I have been doing mutation accumulation experiment in 3 parallel lineages in bacteria and I now want to calculate the...

05 December 2019 5,648 4 View

What is the best method for mammalian blood RNA extraction?

Hi everyone, Does anyone have protocol for mammalian blood RNA extraction with Trizol? I have not obtained good results. Thanks

02 September 2019 2,722 3 View

Is anybody know an effective antibody to detect mScarlet-I signals?

Hello everyone! I would like to visualize the expression of my protein with western blot using plant cell-lysate. My plant contains my protein tagged with mScarlet-I. First, I wonder if I can use...

09 July 2019 6,203 2 View

Does anyone having some suggestion to improve Arabidopsis protoplasts transfection using the Tape-sandwich method?

Hello everyone! I am a student and I am trying to express fluorescently tagged proteins in Arabidopsis using the Tape-Arabidopsis Sandwich method. I isolated and got nice intact protoplasts....

27 April 2019 5,845 3 View

How to separate two organic salts?

I would like to know how can I separate two organic salts which I have in the same sample. I want to purificate both and characterize but I dont know how could I do it Another question is how to...

23 October 2018 4,429 3 View

References about static holds as a method of PAP?

Hi all, I'm currently in the process of looking for references about static holds (for example in squat or bench press) as a method for Potentiation Post Activation or improvement for a heavy...

08 September 2018 2,298 2 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View