I am planning to run some of the PCR products on a gel to identify the products of interest, perform a gel extraction, My products of interest will be approximately around 2300bp in size. In order to get a band with a good resolution I have decided to use TAE and 1% of agarose. I'd also like to know the optimum voltage and time to separate my products (2300 bp) in either 0,8 % or 1% agarose gel. Please assist with the numbers.
How to determine the optimum voltage and time for a better separation in gel electrophoresis?