Hello It's unclear to me the concept of IVCC(integral viable cell concentration) and I'm struggling to calculate them. I have sampled cultured cell in day1 , day3, day5, and day7, and their viable cell concentrations are 5.60*10^5, 2.79*10^6, 4.47*10^6, and 7.24*10^6 respectively(cells/mL). In this case, how to calculate IVCC on each day? Your help is appreciated. Thanks
Miguel Fernández-Niño
IVCC2(E6cells·day/ml)=IVCC1+(VCC1+VCC2)/2*(t2-t1)/1000000
VCC—Viable cell concentration，cell/ml
t1,t2—two different time points,day
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Pranita Kamble Waghmare
You can have MTT assay to calculate the number of viable cell. For that you can take OD at 0 day and subsequently as per your design. The MTT assay is based on the number live cells.
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