Hello everyone, I am currently make a stock and providing research samples in my workplace, I would like to ask your experties what are the best culture media you used to isolate healthy and viable cells. Any hidden technique to maintain its healthy viability? Your knowledge you share will benifit to everyone around. Really appreciate and thanks a lot in advance.
As a matter of fact, best culture media for cell lines relies upon the root of your cell lines can guide you well toward the appropriate media because the demands of cultured cells depend on cell type and the culture’s purpose. The media enhance further specialized and customized according to the demands of that special cell line, and even the particular protein they proving, A general type of medium utilized for general maintenance of adherent mammalian cell cultures in Dulbecco's Modified Eagle Medium (DMEM), For less specialized cultures, a common practice is to use a general medium, such as DMEM, and supplement it with factors that support the cells. For instance, numerous specialists supplement their fundamental DMEM with (FBS) serum gives a ton of supplements and in addition certain connection highlights.
It depends on the cell line you want to culture.
Most often DMEM with 10% serum should be fine.
Hi Thadeo, follow the cell culture protocol provided by the supplier.
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