What kind of contamination can this be?

01 July 2020 5,790 2 View

How to choose the right distance method ?

Hi everyone, I have some issues to determine how to pick correctly the right distance calculation method in R. I have two datasets : one containing habitat variables (worldclim data, so...

15 October 2019 7,562 5 View

How can I dry glycerol on nitrocellulose membrane without damaging antibody?

20 April 2017 793 1 View

High fidelity PCR : experimental problem and enzyme choice ?

15 December 2016 5,142 4 View

When should I block my nc membrane with BSA?

I am creating a sample test of a LFA on a nitrocellulose membrane. I have seen some contradicting protocols regarding blocking the membrane so far. Some suggest doing it before I spot the membrane...

16 April 2016 5,456 2 View

At what setting should I sonicate my carbon nanoparticles?

I will be using the M220 or E210 Covaris sonicator. Any insight would be greatly appreciated! I will be sonicating 1 mg of carbon nanoparticles in 0.1 mL of water and then later with added borate...

14 April 2016 245 3 View

How do I create Washing Buffer (5 mM BB, 1% (w/v) BSA and Storage Buffer (Sb, 100 mM BB, 1% (w/v))?

I already have the 5 mM Borate Buffer and the 100 mM Borate Buffer. I also have 10% BSA. How do I create 5 mL of the WB and 5 mL of the SB? I need these two buffers to couple carbon nanoparticles...

12 April 2016 1,659 3 View

How should I dilute my antigen and antibodies for my test and control line on LFA?

I need to pipet a low molecular weight antigen (around 20 kDA) on my test line and a secondary antibody of anti-rabbit goat antibody- IgG (around 120-160 kDA) on my control line. The...

12 April 2016 8,828 3 View

Is it possible to conjugate antibodies to Acid Black 48 Dye?

I know that because Acid Black 48 is cationic and the antibody is anionic, those two could non-covalently bind. Would this hypothetically work by allowing them to bind through mixing at 4 degrees...

10 April 2016 9,010 2 View

My antibody contains 0.01% sodium azide. Would it be acceptable if I biotinylated the antibody with this concentration of sodium azide?

10 April 2016 9,086 3 View

Heat exchanger with liquid Nitrogen as coolant?

I want to cool Natural Gas from 195 Kelvin to 138 Klevin in a heat exchanger by using liquid nitrogen as the coolant medium. When entering the heat exchanger, the liquid nitrogen will have a...

01 March 2021 6,450 5 View

How to get/count refractive index in gasses?

20 February 2021 8,202 6 View

How to make Fast Green FCF from powder?

Hi everyone We need Fast Green to electroporate plasmid into mice embryos, therefore we simply need it to see some color in the liquid we are electroporating so that electroporation is more...

07 February 2021 1,671 2 View

Which is the best software for the simulation of tribological properties of materials?

I want to simulate the tribological properties of solids, liquids, and fluids, please suggest good software.

02 February 2021 3,947 3 View

Does anybody work with CoolLED in fluorescence microscopy?

I have trouble with exciting wavelengths higher than 460 nm while e.g. ex with 405 nm works. Filtercubes are correc, as I used them with my old xenon lamp before... I work on axiovert200M and with...

25 January 2021 5,201 2 View

How to prepare a liquid sample for FTIR spectrum?

I have a ethanol-water 50% extract of mango peel. I purified mangiferin from the extract with filtration and precipitation. so i obtained a low concentrated extract which i want to se the IR...

24 January 2021 8,880 7 View

How to remove BF3 etherate from a reaction mixture?

20 January 2021 6,322 2 View

Why high voltage and current (and some gases) Cause Spray-mode metal transfer in Gas-Metal Arc Welding (GMAW)?

This transition phenomena of Globular to spray mode as voltage , and especially current is raised, is very commonly known to Manual Metal Arc Welding (MMAW) and Gas-Metal Arc Welding (GMAW)...

20 January 2021 7,984 2 View

Can you have pressure inlet and velocity inlet at the same time?

I was reading a reference (Reitz-A Photographic Study of Flash Boiling Atomization). In his experiment, his boundary condition is delta p and constant velocity. The liquid injection pressure was...

18 January 2021 842 9 View

How can you confirm new liquid FTIR cell fits calibration?

We have an FTIR instrument which was calibrated in transmission/absorption mode using a set of cells for different ranges of D2O concentration. One of the cells at high range broke and the new one...

16 January 2021 4,791 2 View