I have only read a few papers where classical markers of cholinergic neurons are mentioned but not shown. I want to differentiate SH-SY5Y cells to cholinergic neurons with RA and BDNF.
What would be the best marker to confirm that they are cholinergic neurons? ChAT? HB9 and ISL1?
Dear Franco Campos
If you're looking to confirm the cholinergic phenotype of differentiated SH-SY5Y cells, one reliable marker to consider is Choline Acetyltransferase (ChAT). ChAT is an enzyme involved in the synthesis of acetylcholine, a neurotransmitter associated with cholinergic neurons. Immunostaining for ChAT can be a robust method to identify and confirm the presence of cholinergic neurons in your differentiated cell culture. Additionally, you may want to check for other markers associated with cholinergic neurons, such as vesicular acetylcholine transporter (VAChT) or nicotinic acetylcholine receptors. Moreover, You can also check morphological changes and MAP2 expressions
Kind regards
AB Bayazid
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