Hi, I have a simple question to which I seem having problems finding an answer online. Does MG-132 inhibit β5i (inducible) subunit of proteasome?
thank you for any help!
Full information about Proteasome inhibitors, and their respective action sites .... seems to be well described in that review :
Proteasome inhibitors : from research tools to drug candidates
Alexei F. Kisselev*, Alfred L. Goldberg
Chemistry & Biology 8 (2001) 739^758
Best regards. Philippe Chadebech
Yes, I have seen that one but it only refers to Chymotrypsin-like activities which is not really an answer (but some sort of it since both proteasomes have beta 5 subunits). I was thinking about simple experiment published somewhere where purified proteasome was incubated with MG-132.....
Anyway, thank you
In De Bruin & Overkleeft, Angew Chem 2016, they do a bunch of experiments with selective probes for the proteasome subunits, and show inhibition with a bunch of inhibitors (Figure 4), but MG132 is lacking. In Florea & Overkleeft, Chem Biol 2010, Figure 4, you may find what you are looking for (competition of beta5i & beta1, which don't resolve on gel, by MG132).
Thank you, getting closer to the answer but it seems I have to do it myself in the end. I know how to do it but wanted to avoid the experiment itself;)
Michal
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