Hi,
I'm currently having problems with Western blots using the following antibodies from Cell Signaling:
Phospho-Stat3 (Tyr705)
STAT3 (total)
Phospho-Stat5 (Tyr694)
STAT5 (total)
They seem to work very inconsistently, sometimes giving no signal at all. They used to work better for the first 6 months, giving the correct bands. It's not a technical issue - I'm regularly running multiple blots with ~30 different primary Abs, and they work consistently. Ponceau and GAPDH indicate the presence of protein. It's also not an issue of molecular weight - other 100-200 kDa proteins are detected without problems.
I would like to try out some new antibody clones, which ones would you recommend?
I would propose Abcam products, such as anti-STAT5 antibody (cat# ab126832) and/or anti-STAT3 antibody [9D8] (cat# ab119352).
Thank you, and for the phospho forms? Also abcam? They are the most difficult to work with.
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