i am working in HbA1c biosensor modifications, and i need a protein standard for my experiments both glycated and non-glycated.
i need a kit or manual procedure for the isolation of protein to use it as standard.
Hi Abar, I recommend Exocell
http://www.exocell.com/reagents.html
for glycated Hb standards.
Glycated proteins can be isolated using immobilized boronic acid. These are available from many companies including Fisher
https://tools.thermofisher.com/content/sfs/manuals/MAN0011297_Immobil_Boronic_Acid_Gel_UG.pdf
thank you so much, Steingrimur Stefansson
is that Glycated standard blood or pure protein (i don't want blood and all standard i saw it commercially are blood)
and great for that acid, I will try to order it and do the isolation
thank you again
The way Hot pressing can be employ for thermoplastic multilayer, any such process or techniques for thermosets as well?
29 May 2024 504 3 View
Should I go for kjeldahl method or Ion chromatography ?. or any other methods which is more reliable.
22 February 2024 1,025 2 View
If yes how to prepare sample for it ? Should I place the hydrogel pellet under XRD ?
18 February 2024 7,198 3 View
Respected expert, as I have done antioxidant assay for zinc oxide nanoparticle using medicinal plant Serriphidium kurramense. In this assay I have used Ascorbic acid as standard and DPPH as free...
23 November 2023 2,568 2 View
when we do synthesis of nanoparticle through Green route, we always notice the initial color change by adding Plant Extract and the Salt like Zinc acetate in ZnO NPs etc. and if the color change...
16 November 2023 1,742 2 View
I have used different concentrations like I used 1/ 1mg ZnO NPs in 1ml DH2O, Peak "206nm 2/ 1mg ZnO NPs in 3m DH2Ol, peak "220nm" then I used 3/ 1mg ZnO NPs in 5ml DH2O , Peak "220nm" so what I do...
11 November 2023 9,735 2 View
I have done the procedure many time for ZnO NPs synthesized via Green synthesis method from leaf extract but I didn't get the desire peak for UV absorption mentioned in literature that is from...
09 November 2023 2,671 0 View
I have already used two concentrations, i,e: 1: Took 1mg ZnO NP in 1ml water 2: Took 1mg ZnO NP in 3ml water After taking these two concentrations, properly dispersed NP using Ultrasonic...
06 November 2023 9,847 3 View
Greetings to all. Currently, I have successfully purified the GST-tagged fusion protein. The protein degrades as soon as I remove the tag. It is an inherently disordered membrane protein. Please...
25 September 2023 6,803 1 View
Monitoring of fabric material is essential for the creation of textiles. Defective textiles have cost the apparel business a lot of money. In less developed nations like Bangladesh, most of the...
31 August 2023 2,100 1 View
Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)
05 August 2024 9,537 2 View
Are there any statistical methods to justify your sampling technique using SPSS or AMOS?
05 August 2024 9,153 4 View
Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...
05 August 2024 3,783 2 View
Better ways to analyze the qualitative and quantitative data in a sequential explanatory mixed method approaches
04 August 2024 2,703 6 View
In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...
01 August 2024 4,403 4 View
Just bounced on me. Before statistically analysing significant difference, shouldn't we see if data fits normal distribution first? Is 3 replicates enough to testify the hypothesis of normal...
31 July 2024 8,141 13 View
I want to perform a CRISPRa/i library screening for candidate regulatory factors that affect pathogenic Th17 differentiation in PBMC. But I am wondering how to define the size of gene library, so...
31 July 2024 2,150 0 View
I am currently considering a research project focusing on a comparative analysis of starch metabolism in orchids and roses. I am particularly interested in identifying the types and quantities of...
30 July 2024 4,267 2 View
Can an analytical method's limit of quantification (LOQ) be outside its linear dynamic range, or is it always required to be within it? Please provide a thorough explanation supported by verified...
29 July 2024 7,198 9 View
Employing a pragmatic inquiry research design, looking for published research using this method, employing qualitative research data collection methods of semi-structured interview and focus...
28 July 2024 540 2 View